Department of Physiology and Pharmacology
Hillary B. Michelson, PhD
Physiology and Pharmacology
Tel: (718) 270-3182
Structural and Functional Organization of the Hippocampal Inhibitory Circuit
My research interests include: the morphology and synaptic connectivity of the inhibitory circuit in the hippocampus; the study of interneuron-to-interneuron and interneuron-to-principal cell communication pathways; differentiation of interneuron subtypes in the hippocampus; GABAergic neurotransmission within the hippocampus as it relates to epilepsy; maturation of the inhibitory circuit in the hippocampus.
The hippocampus plays an essential role in many normal physiological functions, such as information processing, learning and memory formation, as well as in several pathophysiological conditions, such as epilepsy and Alzheimer's disease. Although much of our current knowledge regarding the organizational structure and function of the hippocampus has been derived from studies of the principal cell population, it has become increasingly clear from recent work that inhibitory interneurons play an integral role in the regulation of hippocampal network activity. Neuronal population oscillations, synchronization, and synaptic plasticity are all influenced by GABAergic inhibition. Thus, an understanding of the structure and function of the inhibitory circuit is essential for a full understanding of neuronal population behavior and network activity, in the hippocampus in particular, and the cerebral cortex overall.
Our laboratory has been investigating the structure and synaptic organization of the inhibitory circuit in the hippocampus. We have been using primarily electrophysiological techniques, combined with anatomical methodologies, to study the morphology and synaptic connectivity of the interneuron population.
Hippocampal interneurons are the GABAergic cells which, when activated, release GABA onto synaptically connected principal cells (primarily the pyramidal neurons of Ammon's horn) to produce a membrane hyper-polarization. We began our investigations by examining the connectivity of interneurons within this inhibitory circuit. Our initial studies addressed the functional aspects of interneuron-to-interneuron communication. These studies indicated that there is recurrent connectivity among interneurons; that is, that interneurons have the ability to excite other interneurons, and that this excitation is mediated by a depolarizing synaptic action of GABA on GABAA receptors.
Our subsequent studies have continued to investigate the organization of the inhibitory circuit of the hippocampus. We have since demonstrated that multiple subpopulations of interneurons exist in the hippocampus. These interneuron subtypes can be differentiated by their recurrent connectivity, by the presence or absence of electrotonic coupling, by their responsiveness to GABAA receptor blocking agents, and also by their output properties. Currently, we are focusing our studies on the output functions of interneurons within the hippocampal circuit, and examining differential interneuronal synaptic outputs onto the individual principal cell populations.
Our most recent studies have demonstrated that, under conditions of hypersynchronous neuronal firing into the hippocampus, the output of interneurons onto dentate granule cells can be excitatory, and can facilitate burst firing in these cells. Our research suggests that synaptically released opioid peptides, possibly co-released with GABA from interneurons, contribute to the development of burst firing in granule cells. We hope that our research into the structure and functional organization of the inhibitory circuit will better our understanding of the important role of the inhibitory circuit in the regulation of normal and abnormal hippocampal function.
Yang Qizong, M.S., Graduate Student
Coordinator and instructor, Neuropharmacology Review for Neurology Residents, St.
Vincent's Hospital, New York
Reviewer for various scientific journals and funding organizations (including N.I.H)
- Michelson, H. B., and Wong, R. K. S. (1991). Excitatory synaptic responses mediated by GABA receptors in the hippocampus. Science 253, 1420-1423.
- Michelson, H. B., and Wong, R. K. S. (1991). Synchronization of GABAergic interneurons in the guinea pig hippocampus in vitro. J. Physiol. 438, 259P.
- Michelson, H. B., and Wong, R. K. S. (1994). Synchronization of inhibitory neurones in the guinea-pig hippocampus in vitro. J. Physiol. 477, 35-45.
- Forti, M., and Michelson, H. B. (1997). Novel glutamate and GABA-independent synaptic depolarization in granule cells of guinea pig hippocampus. J. Physiol. 504, 641-648.
- Forti, M., and Michelson, H. B. (1998). Synaptic connectivity of distinct hilar interneuron subpopulations. J. Neurophys. 79, 3229-3237.