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Department of Pathology

Confocal Microscopy Shared Research Facility
Department of Pathology
Basic Sciences Building Room 4-130

 

Olympus FluoView FV1000 Confocal Laser Scanning Biological Microscope built on the Olympus IX81 Inverted Microscope

The Olympus FV1000 is a fully integrated confocal system configured using the Olympus IX81 inverted microscope and permitting experiments ranging from morphology on fixed cells and tissues to real-time in vitro studies. Images can be exported in TIFF and JPG formats, and analytical graphs can be exported into Microsoft Excel format.

The types of experiments most appropriate for this instrument include slides with fluorescent labels on fixed cultured cells or tissue sections that require high resolution imaging; samples up to 100µm thick; short- and long-term live cell imaging; multiple time point (“mark and find”) studies; and studies requiring image tiling.

The diode lasers have emission lines at 405nm, 473nm, 559nm and 635nm and, with appropriate filters and detectors, allow for the excitation of many commonly-used fluorescent probes.

The Olympus IX81 inverted microscope is equipped with a Prior motorized stage and a Precision Plastics Incubation System that controls temperature, humidity and CO2 for longer live-cell experiments. The chamber can be completely opened for use with samples on slides.

Please note that because the Olympus IX81 microscope is an INVERTED microscope, culture dishes, chambers, and well plates must contain a 0.16-0.19 mm (No. 1.5) glass coverslip for optimal imaging on this microscope. For more information, see companies such as Mat-tek, Inc., that may supply free samples of dishes incorporating coverslips.

For more detailed information on the confocal system or to schedule time, please contact our confocal support specialist, Bill Oxberry (x4472), and complete the Confocal System User Information Form (you will be asked to provide an account number for billing purposes).

As a courtesy to other investigators, users are asked to contact Bill Oxberry as soon as possible if they must cancel their imaging time. A fee ($20) may be assessed if notice of cancellation is not received at least one (1) hour before the reserved time. If the reservation time begins at 9:00 10:00 a.m., cancellation must be received by 4:00 p.m. the afternoon before. Anyone who wishes to image without assistance must be trained and certified by Bill Oxberry.

Notice: The Confocal Microscopy Shared Research Facility will no longer maintain an archive of user images and/or data. Approximately 30 days after an imaging session or experiment, the images and data will be deleted from our system.