Department of Pathology
Confocal Microscopy System Core Facility
Department of Pathology
Basic Science Building Room 4-130
Bio-Rad MRC-1024 Krypton/Argon Confocal Imaging System
and Olympus IX70 Inverted Microscope
The MRC 1024 krypton/argon confocal imaging system from Bio Rad Laboratories, Inc. (MRC 1024) is a fully integrated system configured using an Olympus IX70 inverted microscope. This system allows the definition of multiple regions of interest and optical sectioning through point scanning. Integrated functions include two- and three-dimensional analysis, image statistics, and three-dimensional visualization and segmentation.
The 15mW krypton/argon laser has emission lines at 488, 568 and 647 nm and, with appropriate filters and detectors, allows for the excitation of several commonly-used fluorescent probes (fluorescein, rhodamine, Cy 5, etc.) as well as AlexaFluor tags. The system is equipped with three detector channels, allowing for simultaneous 24-bit acquisition of signals from three separate fluorophores.
In addition to confocal/laser illumination, the Olympus IX70 inverted microscope is equipped for standard fluorescent and brightfield illumination.
To schedule time on the confocal system or for more information, please contact our confocal support specialist, Bill Oxberry (x4472), and complete the Confocal System User Information form (including an account number for billing purposes).
Confocal use with assistance $50/hr
Confocal use without assistance $30/hr
Training on confocal system $50/hr
Cancellation/no-show fee $20
Transfer images to CD Included in user fees
A fee will be assessed if notice of cancellation is not received at least one (1) hour before the reserved time. If the reservation time begins at 9:00-10:00 a.m., cancellation must be received by 4:00 p.m. the afternoon before. Anyone who wishes to image without assistance must be trained by Bill Oxberry.
Any questions regarding use of the system should be directed to Bill Oxberry, MS (718-270-4472; email: William.Oxberry@downstate.edu).