[October 7, 2013]
New Findings Identify Stress Steroid Mediated Withdrawal Anxiety in Dependent Rats:
Reversible by Flumazenil
Brooklyn, NY – Sheryl Smith, PhD, professor of physiology and pharmacology at SUNY Downstate Medical Center, has published new findings demonstrating a reproducible pathology that may help shed light on anxiety and mood volatility in methamphetamine dependence. In her animal study, Dr. Smith demonstrates that neuroactive stress steroids can trigger identifiable changes to the brains of methamphetamine dependent rats in withdrawal. Interestingly, Dr. Smith notes, these changes appear to be reversible by an existing pharmacological agent – flumazenil. The paper, titled, "A Stress Steroid Triggers Anxiety via Increased Expression of a4bd GABAA Receptors in Methamphetamine Dependence," was recently published online in Neuroscience.
"Methamphetamine is an addictive stimulant drug. Dependence on this drug is difficult to treat because of the severity of the symptoms of methamphetamine withdrawal," said Dr. Smith. "In addition to drug craving and lethargy, withdrawal from methamphetamine is associated with stress-triggered anxiety that may compromise therapeutic intervention. In our recent publication, we show that the system that provides inhibitory control in the brain is dysregulated during methamphetamine dependence in laboratory rodents."
The novel inhibitory receptor that is increased by exposure to methamphetamine is also the target for a stress steroid that produces anxiety during withdrawal from methamphetamine. This receptor is also a novel target for flumazenil, a drug commonly used to treat tranquilizer roverdose. Flumazenil reduced expression of the inhibitory receptor and also prevented the anxiety triggered by the stress steroid during methamphetamine withdrawal. These findings suggest a novel mechanism for stress-triggered anxiety in methamphetamine dependence where flumazenil may have important therapeutic value.
One component of the stress response is the release of the steroid allopregnanolone or THP. This steroid modulates GABAA receptors, with α4βδ GABARs the most sensitive target. These receptors are extrasynaptic and generate a tonic current that is neuroprotective. They also exhibit a high degree of plasticity in response to increases in neuronal excitability, which would accompany METH exposure. In some cases, flumazenil was administered during the 24 hour withdrawal period. Although this drug is a benzodiazepine antagonist, it also binds to α4βδ GABARs and has been shown to regulate α4 expression.
The study results show that chronic METH treatment and its withdrawal significantly increased expression of α4 and δ GABAR subunits by 2 to 3-fold. This effect was prevented, however, by flumazenil administration during the withdrawal period.
Dr. Smith continued, "Our results suggest that increased α4βδ GABARs mediate the anxiety response to stress steroids after METH withdrawal, an effect prevented by flumazenil. Because stress-triggered anxiety can result in drug relapse, flumazenil may have important therapeutic benefit in METH dependence."
The work in this study was supported by grants from the U.S. National Institutes of Health: DA09618, AA12958 and MH100561, and was also supported by a contract from Hythiam, Inc.
SUNY Downstate Medical Center, founded in 1860, was the first medical school in the United States to bring teaching out of the lecture hall and to the patient's bedside. A center of innovation and excellence in research and clinical service delivery, SUNY Downstate Medical Center comprises a College of Medicine, Colleges of Nursing and Health Related Professions, a School of Graduate Studies, a School of Public Health, University Hospital of Brooklyn, and an Advanced Biotechnology Park and Biotechnology Incubator.
SUNY Downstate ranks ninth nationally in the number of alumni who are on the faculty of American medical schools. More physicians practicing in New York City have graduated from SUNY Downstate than from any other medical school.