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MASS SPECTROMETRY CORE FACILITYDepartment of Biochemistry, Box 8
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Julie I. Rushbrook, Ph.D. |
Jimmy R. Calaycay, M.S. |
Consultation with Dr. Rushbrook or Mr. Calaycay is required prior to preparation and submission of samples.
ThermoFinnigan LCQDECA XP Ultra Sensitive Ion Trap Mass Spectrometer; Waters 626 LC System with Waters 996 Photodiode Array Detector.
Samples are analyzed in the order in which they are submitted. Completion time varies with workload and is usually 3-10 days.
Charges are for faculty of Downstate Medical Center and affiliated institutions.
Outside investigators at nonprofit organizations will incur a 25% surcharge, those at for-profit institutions a 50% surcharge.
There is a 25% volume discount when 10 or more samples are submitted at one time.
| Services | Charges | |
|---|---|---|
| CONSULTATION:: | ||
| Required prior to preparation of samples for analysis. Call either number above for appointment. |
No charge | |
| STAND-ALONE HPLC: | ||
| • HPLC run by Core Facility | $45 / hr | |
| • Training to run HPLC | $45 / hr | |
| • HPLC run by investigator's laboratory (supply own solvents) | $100 / day | |
| PROTEIN STUDIES: | ||
| Proteins may be provided in solution (documention of purity required, e.g., SDS-gel or HPLC chromatogram), or as a band in a gel. For many purposes a gel band is easier to work with. | ||
| Clean-up / desalting of protein solution samples by: | ||
| • Sep-Pak | $16.00 | |
| • Zip-Tip | $6.00 | |
| Intact protein mass determination by : | ||
| • ESI-MS (pure protein) | $60.00 | |
| • LC-MS | $100.00 | |
| Protein identification by sequencing of peptides generated by digestion: | ||
| • Digestion of protein in solution. | $25.00 | |
| • Reduction/alkylation/digestion of protein in solution or in gel piece. | $75.00 | |
| • Peptide sequencing by LC-MS/MS of the digest (includes 2 hrs manual data analysis sufficient for straight-forward sample). |
$240.00 | |
| • Additional manual analysis / hr, up to 6 hours. (No charge for further data analysis.) | $45 / hr | |
| Post-translational modification studies: | ||
| Post-translational modification studies are challenging. An approach will be approved by the P.I. Charges for procedures chosen will be as above. Steps may include: | ||
| • Mass determination of protein in solution | ||
| • Reduction/alkylation/digestion | ||
| • Comparative HPLC | ||
| • Sequence information from LC-MS/MS of digest | ||
| • Charge for extended data analysis beyond 8 hours | ||
| SMALL MOLECULE STUDIES: | ||
| Clean-up / desalting by: | ||
| • Sep-Pak | $16.00 | |
| • Zip-Tip | $6.00 | |
| Sample handling costs: | ||
| Sample handling required prior to analysis will be charged according to reagent cost and time involved. | ||
| Mass determination by: | ||
| • ESI-MS (fairly pure sample) | $60.00 | |
| • LC-MS (complex sample) | $100.00 | |
| Structure determination by: | ||
| • ESI-MS/MS | $110.00 | |
| • ESI-MS/MS/MS | $110.00 | |
| • LC-MS/MS | $150.00 | |
| • LC-MS/MS/MS | $150.00 | |
Many ongoing projects, especially those involving small molecules, are custom analyses which required development.
Development may take from a few days to several weeks.
There will be a charge of $270 / day (6 hours at $45.00 /hr) for each of the first 5 days spent in development. There will be no charge for further time. The P.I. will purchase reagents and test molecules specific to the analysis. If the analysis is not possible, charges wil be limited to 3 day's work.
Please use the following format to acknowledge work done by the facility:
"Type of work, e.g., Protein identification by mass spectrometry") was carried out by the Mass Spectrometry Core Facility at SUNY Downstate Medical Center, Brooklyn NY 11203".
Text appropriate for Methods, Results and Discussion Sections, as well as figures, will be provided upon request. Relevant portions of manuscripts and grants can be submitted for review. Please provide these in a timely manner.
When appropriate, the principal investigator is encouraged to acknowledge original contributions such as development of novel methods, analysis of complex data or special insights, with co-authorship.
