TRANSGENIC MOUSE CORE FACILITY
Services
Production of Transgenic Mice
- Pronuclear injection of embryos with conventional or BAC transgenes
- Embryo transfer to pseudopregnant females
- Lentiviral infection and transfer of embryos
Rederivations
- Frozen embryo transfers
- New embryo transfers
Consultation
- Design of transgenic vectors
- Preparation of transgenic DNA
- Genotyping
Contacts
Christopher Roman, Ph. D., Director
Asst Professor, Microbiology and Immunology
(718) 270-1310
Christopher.Roman@Downstate.edu
Chongmin Huan, M.D., Ph. D., Research Scientist
Vol Asst Professor, Microbiology and Immunology
(718) 270-4570
Chongmin.Huan@Downstate.edu
Contact Dr. Roman for fees or to make an appointment for a consult. The P.I. must have an IACUC approved animal protocol to use mice for experimental purposes.
Pronuclear Microinjection Sublicense Agreement
Methods of pronuclear microinjection are patented under United State Patent No. 4,873,191, entitled "Genetic Transformation of Zygotes," assigned to Ohio University and licensed to Xenogen Biosciences. The State University of New York (SUNY) currently holds a Sublicense Agreement, which provides for the generation of transgenic animals for SUNY faculty, as well as for individuals at other organizations. For individuals external to the SUNY, a specific MTA form must be completed before service can be provided; please contact John Allen john.allen@Downstate.edu for this form.
