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Department of Cell Biology Faculty

Andrey Pisarev

Andrey Pisarev, Ph.D.

Phone: 718-270-1143

e-mail: Andrey.Pisarev@downstate.edu

Research Interests

Protein synthesis, an essential cellular process, is divided into four stages: initiation, elongation, termination and ribosome recycling. The mechanisms of the last two stages, termination and recycling, have been described only recently as a consequence of advances that permitted all stages of translation to be reconstituted in vitro from individual translational components: 40S and 60S ribosomal subunits, aminoacyl-tRNA, mRNA and translation factors. However, despite the fact that all factors required for the translation cycle have been identified, and their principal roles have been determined, the molecular mechanisms of many fundamental steps in translation remain unknown. The possibility to reconstitute the entire eukaryotic translation cycle in vitro from individual components permits the analysis of basic and regulatory molecular mechanisms of individual steps, and our laboratory employs this approach in conjunction with a variety of biochemical and biophysical techniques to address poorly characterized aspects of translation in higher eukaryotes.

Training
  • 2000-2003 PhD student, Department of Chemistry, Moscow State University, Moscow, Russia
  • 2004-2009 Postdoctoral Fellow, Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY
  • 2009 - Assistant Professor, Department of Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY
Recent Publications
  1. Pisareva VP & Pisarev AV (2016) DHX29 and eIF3 cooperate in ribosomal scanning on structured mRNAs during translation initiation. RNA 22: 1859-1870
  2. Pisareva VP & Pisarev AV (2016) DHX29 reduces leaky scanning through an upstream AUG codon regardless of its nucleotide context. Nucleic Acids Res. 44: 4252-4265
  3. Pisareva VP, Muslimov IA, Tcherepanov A & Pisarev AV (2015) Characterization of Novel Ribosome-Associated Endoribonuclease SLFN14 from Rabbit Reticulocytes. Biochemistry 54: 3286-s3301
  4. Pisareva VP & Pisarev AV (2014) eIF5 and eIF5B together stimulate 48S initiation complex formation during ribosomal scanning. Nucleic Acids Res. 42: 1205212069
  5. Pisareva, V.P., Skabkin, M.A., Hellen, C.U., Pestova, T.V. and Pisarev, A.V. (2011) Dissociation by Pelota, Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes. EMBO J., 30, 1804-1817.