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j.mcphee

Joseph R. McPhee

Asst. Professor Dept. of Biochemistry, Research
Asst. Prof. And Director of Research Dept. of Otolaryngology
Ph.D in Biology NYU 1984




Molecular Genetics of Human Keloid Formation

Keloids are disfiguring scars that proliferate beyond the boundaries of the original site of cutaneous injury . Keloids can continue to grow indefinitely and no satisfactory treatment has been identified. Etiological evidence has pointed to a possible genetic predisposition in humans. However, it is unclear what the inheritance patterns of this disease are. Various studies have provided conflicting evidence, supporting an autosomal recessive pattern, an autosomal dominant pattern, or an autosomal dominant with incomplete penetrance. The greatest occurrence of keloid formation is among the female population, although the statistical evidence is obscured due to the number of females forming keloids following ear-piercing.

In vitro studies of keloid fibroblasts have implicated several cytokines in the pathogenesis of keloid formation, including transforming growth factor beta (TGF-b), epidermal growth factor (EGF), fibroblast growth factor (FGF), and platelet derived growth factor (PDGF). TGF-b has received the most attention of all the cytokines due to its ability to promote the synthesis of collagen, which is the principal constituent of the keloid mass. Three different isoforms of TGF-b have been identified (b1, b2 and b3), with each isoform performing a different biological role in the wound healing process. According to western blot studies, keloid fibroblasts have increased amounts of the TGF-b1 and TGF-b2 cytokine present compared to normal dermal fibroblasts. Our research will use a procedure known as gene screening to determine whether any of the cytokines mentioned above result in a pattern of expression that could lead to keloid formation and to determine if there is a simple method of intervention that could prevent this from occurring.

 

PUBLICATIONS:

Har El, G., McPhee JR. Transcranial Magnetic Stimulation in Facial Nerve Injury. In Update on Facial Nerve Disorders (C. Shelton Ed.) 2001, 43-50

Frenz, DA, McPhee JR, Van de Water, TR. Structural and Functional Development of the Ear. In Physiology of the Ear (AF Jahn and J Santos-Sacchi Ed.) 2001, 191-214

Har El, G. and McPhee JR. Transcranial magnetic stimulation in acute facial nerve injury.Laryngoscope. 2000 Jul;110(7):1105-11.

McPhee, JR, Gordon, MA, Ruben RJ, Van De Water TR. Evidence of abnormal stromelysin mRNA expression in suspected carriers of otosclerosis. A possible molecular marker. Arch Otolaryngol Head Neck Surg. 1993 Oct;119(10):1108-16.

Gordon, MA, McPhee, JR, Van de Water, TR, Ruben, RJ. Aberration of the tissue collagenase system in association with otosclerosis.
Am J Otol. 1992 Sep;13(5):398-407.

McPhee, JR, Van De Water, TR, Gordon, MA. Otosclerosis. Correlation of clinical incidence and abnormal TIMP gene expression.
Ann N Y Acad Sci. 1991;630:319-21.