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DNA Sequencing

DNA sequence image

The Department of Anatomy and Cell Biology maintains a DNA sequencing laboratory, based on an Applied Biosystems model 373A automated DNA sequencer. This machine detects fluorescently labeled dideoxy terminators used in a Sanger-type dideoxy sequencing reaction utilizing single stranded (e.g. M13 and asymmetric PCR products) or double stranded (plasmids, PCR products) DNA templates. DNA sequencing is provided on a fee for service basis. DNA samples are usually sequenced within several days. Universal forward and reverse sequencing primers are provided; all other primers must be provided by the user. DNA sequence data is available in both printed and computer form, and can be picked up in person or sent by both electronic and/or regular mail. Help with database searching and analysis is also available.

    DNA TO BE SEQUENCED # BP (99%) COST/SAMPLE
  • Single-stranded 350-450 $ 20.00
  • Plasmid 350-450 $ 20.00
  • PCR DNA 300-350 $ 20.00

Dr. John Lewis
Department of Anatomy and Cell Biology - Box 5
Basic Science Building, Room 2-95 • Tel : 718 270 - 2215
Fax : 718 270 - 3732 • E-mail : jlewis@downstate.edu